A semi-automatic method for extracting mitochondrial cristae ...
WEBMar 28, 2024 · We present a new method for the efficient segmentation and 3D shape analysis of mitochondrial inner components from FIBSEM images using multiplanar UNET and persistent homology.
3D reconstruction of murine mitochondria reveals changes in …
WEBNov 13, 2023 · The 3D reconstruction data, in combination with the prior TEM results, show that mitochondrial dynamics change with the loss of MICOS subunits and mimic the mitochondrial phenotypes observed during aging.
Automated segmentation and tracking of mitochondria in live …
WEBAug 19, 2021 · Here, we introduce Mitometer, an algorithm for fast, unbiased, and automated segmentation and tracking of mitochondria in live-cell two-dimensional and three-dimensional time-lapse images....
Visualizing Mitochondrial Form and Function within the Cell
WEBFor light microscopy, there are three general approaches to visualizing mitochondrial structures: 1) labeling mitochondria in live cells with fluorescent dyes, 2) immunostaining fixed cells with mitochondria-specific fluorescent antibodies, and 3) using genetically encoded fluorescent mitochondrial tags. Using live cell fluorescent dyes to ...
Mitochondria in disease: changes in shapes and dynamics
WEBApr 1, 2024 · 3D electron microscopy is developing as a potential clinical diagnostics tool for exploring mitochondrial pathology. Characterizing mitochondrial structure may elucidate tissue-dependent differences and aid in understanding different cell types and conditions associated with different health outcomes. Abstract.
Quantitative 3D Mapping of the Human Skeletal Muscle Mitochondrial ...
WEBThis study also identifies morphological features that distinguish human from mouse skeletal muscle mitochondria, and healthy individuals from patients with mtDNA mutations, including an elevated frequency of simple mitochondria and mitochondrial nanotunnels in mitochondrial disease.
Current Progress and Challenges in Large-Scale 3D Mitochondria …
WEBIn this paper, we present the results of the MitoEM challenge on mitochondria 3D instance segmentation from electron microscopy images, organized in conjunction with the IEEE-ISBI 2021 conference. Our benchmark dataset consists of two large-scale 3D volumes, one from human and one from rat cortex tissue, which are 1,986 times larger than ...
Returning to the Fold for Lessons in Mitochondrial Crista …
WEBMay 18, 2020 · In this review, Pánek et al. have surveyed crista diversity found in mitochondria from disparate organisms, identifying persistent shapes of cristae and discussing possible molecular mechanisms behind their varied morphology.
Mitopherogenesis, a form of mitochondria-specific ectocytosis ...
WEBNov 9, 2023 · Strikingly, our three-dimensional electron microscopy analyses indicate that mitochondrial quantity requires precise modulation during sperm development, which is critically mediated by...
Machine learning-based 3D segmentation of mitochondria in …
WEBMay 1, 2024 · These results demonstrate that our trained classifier in TWS 3D can faithfully segment 3D mitochondrial networks, while preserving nuanced morphological characteristics in the original images, making discoveries of subtle changes possible.