For decades, researchers have wondered why some people with risky gene variants go on to develop Parkinson’s disease, while ...

A massively parallel CRISPRi screen demonstrated that E. coli metabolism is very robust against knockdowns of enzymes, and multi-omics data revealed the mechanisms behind it.

CRISPRi and CRISPRa technologies allow the user to control transcription using modified forms of Cas9 (Figure 1).In both cases, the catalytically deactivated Cas9 (dCas9), no longer able to cut ...

A massively parallel CRISPRi screen demonstrated that E. coli metabolism is very robust against knockdowns of enzymes, and multi-omics data revealed the mechanisms behind it.

In another bioRxiv preprint uploaded June 22, researchers led by Li Gan at Weill Cornell Medicine in New York, who collaborated with Kampmann, reported a similar CRISPRi screen in iPSC-derived neurons ...

The CRISPRi screen identified several potential targetable regulators of STEAP1, including MYC, SWI/SNF chromatin remodeling complexes. Conclusions: Our study establishes a strong association between ...

The large-scale CRISPRi screen uncovered genes that were essential for both neurons and iPSCs yet caused different transcriptomic phenotypes when knocked down. "For me, one of the most exciting ...

A University of Wisconsin-Madison researcher and his collaborators at the University of California, San Francisco have repurposed the gene-editing tool CRISPR to study which genes are targeted by ...

CRISPRi & CRISPRa Tools for Genome-Wide Screening from thescientistllc on Vimeo. Forward genetic screening with CRISPR-Cas9 has created remarkable new opportunities for biological discovery. The power ...

Enter a CRISPRi-based screen Kampmann previously used to analyze 2D cultures of iPSC-derived neurons (Kampmann et al., 2019). The scientists ran this screen in the iAssembloids, suppressing thousands ...