the bacterial plasmid origin of replication (ORI) functions as a conflicting core promoter and (ii) a type I interferon (IFN-I) response is activated. These cause confounding false positives and ...

Data are shown for DH5α E. coli cells transformed with a plasmid containing Chloramphenicol and Kanamycin resistance genes (p15a ORI). The efficiency of this automated bacterial transformation ...