More information: Sifeng Gu et al, Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens, Nature Communications (2025). DOI: 10.1038/s41467-025-59948 ...

All told, Komor had a library of around 12,000 different guide RNA combinations for the CRISPRi screen. Once they collected all the cells that had fluoresced green, ...

Komor’s CRISPRi cells had two guide RNAs — one to activate the fluorescent protein maker with ... Komor had a library of around 12,000 different guide RNA combinations for the CRISPRi screen.

All told, Komor had a library of around 12,000 different guide RNA combinations for the CRISPRi screen. Once they collected all the cells that had fluoresced green, they sequenced the guide RNAs to ...

The CRISPRi screen identified several potential targetable regulators of STEAP1, including MYC, SWI/SNF chromatin remodeling complexes. Conclusions: Our study establishes a strong association between ...

Background: Inactivating missense mutations in the tumor suppressor gene speckle-type POZ (SPOP) increase the transcriptional activity of the androgen receptor (AR) and sensitivity to hormonal ...

We developed an iPSC-derived microglia-neuron coculture system and high-throughput flow cytometry readout for synaptic pruning to enable parallel CRISPRi-based screening of phagocytosis of beads, ...

Custom R script used to analyze the Illumina NGS dataset following a genome-wide screen of Salmonella Typhimurium (see https://doi.org/10.1101/2024.12.16.628777 for ...

Cas9 screen was performed as the CRISPRi screen above using a Cas9+ iSLK cell line infected with a copy of BAC16 containing a HaloTag-ORF68 fusion. The library was then amplified using staggered ...

This study sets the stage of combining CRISPRi/a screen with iPSC technology for future drug discovery endeavors aimed at eliminating off-target cardiotoxicities of chemotherapy agents. eLetters (0) ...

Before we began to use Anker's screen protectors, amFilm was our go-to brand. It's still a great option if you want something that's more inexpensive while still offering a lot of bang for your buck.